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KMID : 0811720180220050555
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Korean Journal of Physiology & Pharmacology
2018 Volume.22 No. 5 p.555 ~ p.566
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Human umbilical cord blood mesenchymal stem cells engineered to overexpress growth factors accelerate outcomes in hair growth
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Bak Dong-Ho
Choi Mi-Ji
Kim Soon-Re
Lee Byung-Chul
Kim Jae-Min
Jeon Eun-Su
Oh Won-Il
Lim Ee-Seok
Park Byung-Cheol
Kim Moo-Joong
Na Jung-Tae
Kim Beom-Joon
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Abstract
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Human umbilical cord blood mesenchymal stem cells (hUCB-MSCs) are used in tissue repair and regeneration; however, the mechanisms involved are not well understood. We investigated the hair growth-promoting effects of hUCB-MSCs treatment to determine whether hUCB-MSCs enhance the promotion of hair growth. Furthermore, we attempted to identify the factors responsible for hair growth. The effects of hUCB-MSCs on hair growth were investigated in vivo, and hUCB-MSCs advanced anagen onset and hair follicle neogeneration. We found that hUCB-MSCs co-culture increased the viability and up-regulated hair induction-related proteins of human dermal papilla cells (hDPCs) in vitro. A growth factor antibody array revealed that secretory factors from hUCB-MSCs are related to hair growth. Insulin-like growth factor binding protein-1 (IGFBP-1) and vascular endothelial growth factor (VEGF) were increased in co-culture medium. Finally, we found that IGFBP-1, through the colocalization of an IGF-1 and IGFBP-1, had positive effects on cell viability; VEGF secretion; expression of alkaline phosphatase (ALP), CD133, and β-catenin; and formation of hDPCs 3D spheroids. Taken together, these data suggest that hUCB-MSCs promote hair growth via a paracrine mechanism.
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KEYWORD
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Alopecia, Dermal papilla cell, Hair growth, IGFBP-1, Stem cell, Stem-cell therapy
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